<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garg, Shilpa</style></author><author><style face="normal" font="default" size="100%">Fungtammasan, Arkarachai</style></author><author><style face="normal" font="default" size="100%">Carroll, Andrew</style></author><author><style face="normal" font="default" size="100%">Chou, Mike</style></author><author><style face="normal" font="default" size="100%">Schmitt, Anthony</style></author><author><style face="normal" font="default" size="100%">Zhou, Xiang</style></author><author><style face="normal" font="default" size="100%">Mac, Stephen</style></author><author><style face="normal" font="default" size="100%">Peluso, Paul</style></author><author><style face="normal" font="default" size="100%">Hatas, Emily</style></author><author><style face="normal" font="default" size="100%">Ghurye, Jay</style></author><author><style face="normal" font="default" size="100%">Maguire, Jared</style></author><author><style face="normal" font="default" size="100%">Mahmoud, Medhat</style></author><author><style face="normal" font="default" size="100%">Cheng, Haoyu</style></author><author><style face="normal" font="default" size="100%">Heller, David</style></author><author><style face="normal" font="default" size="100%">Zook, Justin M</style></author><author><style face="normal" font="default" size="100%">Moemke, Tobias</style></author><author><style face="normal" font="default" size="100%">Marschall, Tobias</style></author><author><style face="normal" font="default" size="100%">Sedlazeck, Fritz J</style></author><author><style face="normal" font="default" size="100%">Aach, John</style></author><author><style face="normal" font="default" size="100%">Chin, Chen-Shan</style></author><author><style face="normal" font="default" size="100%">Church, George M</style></author><author><style face="normal" font="default" size="100%">Li, Heng</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chromosome-scale, haplotype-resolved assembly of human genomes.</style></title><secondary-title><style face="normal" font="default" size="100%">Nat Biotechnol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nat Biotechnol</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Algorithms</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromosomes, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Haplotypes</style></keyword><keyword><style  face="normal" font="default" size="100%">Heterozygote</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism, Single Nucleotide</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021 03</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">309-312</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Haplotype-resolved or phased genome assembly provides a complete picture of genomes and their complex genetic variations. However, current algorithms for phased assembly either do not generate chromosome-scale phasing or require pedigree information, which limits their application. We present a method named diploid assembly (DipAsm) that uses long, accurate reads and long-range conformation data for single individuals to generate a chromosome-scale phased assembly within 1 day. Applied to four public human genomes, PGP1, HG002, NA12878 and HG00733, DipAsm produced haplotype-resolved assemblies with minimum contig length needed to cover 50% of the known genome (NG50) up to 25 Mb and phased ~99.5% of heterozygous sites at 98-99% accuracy, outperforming other approaches in terms of both contiguity and phasing completeness. We demonstrate the importance of chromosome-scale phased assemblies for the discovery of structural variants (SVs), including thousands of new transposon insertions, and of highly polymorphic and medically important regions such as the human leukocyte antigen (HLA) and killer cell immunoglobulin-like receptor (KIR) regions. DipAsm will facilitate high-quality precision medicine and studies of individual haplotype variation and population diversity.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/33288905?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rochtus, Anne</style></author><author><style face="normal" font="default" size="100%">Olson, Heather E</style></author><author><style face="normal" font="default" size="100%">Smith, Lacey</style></author><author><style face="normal" font="default" size="100%">Keith, Louisa G</style></author><author><style face="normal" font="default" size="100%">El Achkar, Christelle</style></author><author><style face="normal" font="default" size="100%">Taylor, Alan</style></author><author><style face="normal" font="default" size="100%">Mahida, Sonal</style></author><author><style face="normal" font="default" size="100%">Park, Meredith</style></author><author><style face="normal" font="default" size="100%">Kelly, McKenna</style></author><author><style face="normal" font="default" size="100%">Shain, Catherine</style></author><author><style face="normal" font="default" size="100%">Rockowitz, Shira</style></author><author><style face="normal" font="default" size="100%">Rosen Sheidley, Beth</style></author><author><style face="normal" font="default" size="100%">Poduri, Annapurna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic diagnoses in epilepsy: The impact of dynamic exome analysis in a pediatric cohort.</style></title><secondary-title><style face="normal" font="default" size="100%">Epilepsia</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Epilepsia</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adolescent</style></keyword><keyword><style  face="normal" font="default" size="100%">Adult</style></keyword><keyword><style  face="normal" font="default" size="100%">Age of Onset</style></keyword><keyword><style  face="normal" font="default" size="100%">Brain Diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">Child</style></keyword><keyword><style  face="normal" font="default" size="100%">Child, Preschool</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromosomes, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Cohort Studies</style></keyword><keyword><style  face="normal" font="default" size="100%">Epilepsy</style></keyword><keyword><style  face="normal" font="default" size="100%">Epilepsy, Generalized</style></keyword><keyword><style  face="normal" font="default" size="100%">Exome</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Testing</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Infant</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Microarray Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Whole Exome Sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Young Adult</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2020 02</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">61</style></volume><pages><style face="normal" font="default" size="100%">249-258</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;OBJECTIVE: &lt;/b&gt;We evaluated the yield of systematic analysis and/or reanalysis of whole exome sequencing (WES) data from a cohort of well-phenotyped pediatric patients with epilepsy and suspected but previously undetermined genetic etiology.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS: &lt;/b&gt;We identified and phenotyped 125 participants with pediatric epilepsy. Etiology was unexplained at the time of enrollment despite clinical testing, which included chromosomal microarray (57 patients), epilepsy gene panel (n = 48), both (n = 28), or WES (n = 8). Clinical epilepsy diagnoses included developmental and epileptic encephalopathy (DEE), febrile infection-related epilepsy syndrome, Rasmussen encephalitis, and other focal and generalized epilepsies. We analyzed WES data and compared the yield in participants with and without prior clinical genetic testing.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS: &lt;/b&gt;Overall, we identified pathogenic or likely pathogenic variants in 40% (50/125) of our study participants. Nine patients with DEE had genetic variants in recently published genes that had not been recognized as epilepsy-related at the time of clinical testing (FGF12, GABBR1, GABBR2, ITPA, KAT6A, PTPN23, RHOBTB2, SATB2), and eight patients had genetic variants in candidate epilepsy genes (CAMTA1, FAT3, GABRA6, HUWE1, PTCHD1). Ninety participants had concomitant or subsequent clinical genetic testing, which was ultimately explanatory for 26% (23/90). Of the 67 participants whose molecular diagnoses were &quot;unsolved&quot; through clinical genetic testing, we identified pathogenic or likely pathogenic variants in 17 (25%).&lt;/p&gt;&lt;p&gt;&lt;b&gt;SIGNIFICANCE: &lt;/b&gt;Our data argue for early consideration of WES with iterative reanalysis for patients with epilepsy, particularly those with DEE or epilepsy with intellectual disability. Rigorous analysis of WES data of well-phenotyped patients with epilepsy leads to a broader understanding of gene-specific phenotypic spectra as well as candidate disease gene identification. We illustrate the dynamic nature of genetic diagnosis over time, with analysis and in some cases reanalysis of exome data leading to the identification of disease-associated variants among participants with previously nondiagnostic results from a variety of clinical testing strategies.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/31957018?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Battle, Alexis</style></author><author><style face="normal" font="default" size="100%">Brown, Christopher D</style></author><author><style face="normal" font="default" size="100%">Engelhardt, Barbara E</style></author><author><style face="normal" font="default" size="100%">Montgomery, Stephen B</style></author></authors><translated-authors><author><style face="normal" font="default" size="100%">GTEx Consortium</style></author><author><style face="normal" font="default" size="100%">Laboratory, Data Analysis &amp;Coordinating Center (LDACC)—Analysis Working Group</style></author><author><style face="normal" font="default" size="100%">Statistical Methods groups—Analysis Working Group</style></author><author><style face="normal" font="default" size="100%">Enhancing GTEx (eGTEx) groups</style></author><author><style face="normal" font="default" size="100%">NIH Common Fund</style></author><author><style face="normal" font="default" size="100%">NIH/NCI</style></author><author><style face="normal" font="default" size="100%">NIH/NHGRI</style></author><author><style face="normal" font="default" size="100%">NIH/NIMH</style></author><author><style face="normal" font="default" size="100%">NIH/NIDA</style></author><author><style face="normal" font="default" size="100%">Biospecimen Collection Source Site—NDRI</style></author><author><style face="normal" font="default" size="100%">Biospecimen Collection Source Site—RPCI</style></author><author><style face="normal" font="default" size="100%">Biospecimen Core Resource—VARI</style></author><author><style face="normal" font="default" size="100%">Brain Bank Repository—University of Miami Brain Endowment Bank</style></author><author><style face="normal" font="default" size="100%">Leidos Biomedical—Project Management</style></author><author><style face="normal" font="default" size="100%">ELSI Study</style></author><author><style face="normal" font="default" size="100%">Genome Browser Data Integration &amp;Visualization—EBI</style></author><author><style face="normal" font="default" size="100%">Genome Browser Data Integration &amp;Visualization—UCSC Genomics Institute, University of California Santa Cruz</style></author><author><style face="normal" font="default" size="100%">Lead analysts:</style></author><author><style face="normal" font="default" size="100%">Laboratory, Data Analysis &amp;Coordinating Center (LDACC):</style></author><author><style face="normal" font="default" size="100%">NIH program management:</style></author><author><style face="normal" font="default" size="100%">Biospecimen collection:</style></author><author><style face="normal" font="default" size="100%">Pathology:</style></author><author><style face="normal" font="default" size="100%">eQTL manuscript working group:</style></author></translated-authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic effects on gene expression across human tissues.</style></title><secondary-title><style face="normal" font="default" size="100%">Nature</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nature</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alleles</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromosomes, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Organ Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">Quantitative Trait Loci</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2017 Oct 11</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">550</style></volume><pages><style face="normal" font="default" size="100%">204-213</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of disease.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">7675</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/29022597?dopt=Abstract</style></custom1></record></records></xml>