<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nattestad, Maria</style></author><author><style face="normal" font="default" size="100%">Goodwin, Sara</style></author><author><style face="normal" font="default" size="100%">Ng, Karen</style></author><author><style face="normal" font="default" size="100%">Baslan, Timour</style></author><author><style face="normal" font="default" size="100%">Sedlazeck, Fritz J</style></author><author><style face="normal" font="default" size="100%">Rescheneder, Philipp</style></author><author><style face="normal" font="default" size="100%">Garvin, Tyler</style></author><author><style face="normal" font="default" size="100%">Fang, Han</style></author><author><style face="normal" font="default" size="100%">Gurtowski, James</style></author><author><style face="normal" font="default" size="100%">Hutton, Elizabeth</style></author><author><style face="normal" font="default" size="100%">Tseng, Elizabeth</style></author><author><style face="normal" font="default" size="100%">Chin, Chen-Shan</style></author><author><style face="normal" font="default" size="100%">Beck, Timothy</style></author><author><style face="normal" font="default" size="100%">Sundaravadanam, Yogi</style></author><author><style face="normal" font="default" size="100%">Kramer, Melissa</style></author><author><style face="normal" font="default" size="100%">Antoniou, Eric</style></author><author><style face="normal" font="default" size="100%">McPherson, John D</style></author><author><style face="normal" font="default" size="100%">Hicks, James</style></author><author><style face="normal" font="default" size="100%">McCombie, W Richard</style></author><author><style face="normal" font="default" size="100%">Schatz, Michael C</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Complex rearrangements and oncogene amplifications revealed by long-read DNA and RNA sequencing of a breast cancer cell line.</style></title><secondary-title><style face="normal" font="default" size="100%">Genome Res</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Genome Res.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Breast Neoplasms</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Amplification</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Rearrangement</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic Structural Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">High-Throughput Nucleotide Sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">MCF-7 Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Oncogenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptor, ErbB-2</style></keyword><keyword><style  face="normal" font="default" size="100%">Repetitive Sequences, Nucleic Acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcriptome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2018 08</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">28</style></volume><pages><style face="normal" font="default" size="100%">1126-1135</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The SK-BR-3 cell line is one of the most important models for HER2+ breast cancers, which affect one in five breast cancer patients. SK-BR-3 is known to be highly rearranged, although much of the variation is in complex and repetitive regions that may be underreported. Addressing this, we sequenced SK-BR-3 using long-read single molecule sequencing from Pacific Biosciences and develop one of the most detailed maps of structural variations (SVs) in a cancer genome available, with nearly 20,000 variants present, most of which were missed by short-read sequencing. Surrounding the important  oncogene (also known as ), we discover a complex sequence of nested duplications and translocations, suggesting a punctuated progression. Full-length transcriptome sequencing further revealed several novel gene fusions within the nested genomic variants. Combining long-read genome and transcriptome sequencing enables an in-depth analysis of how SVs disrupt the genome and sheds new light on the complex mechanisms involved in cancer genome evolution.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/29954844?dopt=Abstract</style></custom1></record></records></xml>