<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Costello, Maura</style></author><author><style face="normal" font="default" size="100%">Fleharty, Mark</style></author><author><style face="normal" font="default" size="100%">Abreu, Justin</style></author><author><style face="normal" font="default" size="100%">Farjoun, Yossi</style></author><author><style face="normal" font="default" size="100%">Ferriera, Steven</style></author><author><style face="normal" font="default" size="100%">Holmes, Laurie</style></author><author><style face="normal" font="default" size="100%">Granger, Brian</style></author><author><style face="normal" font="default" size="100%">Green, Lisa</style></author><author><style face="normal" font="default" size="100%">Howd, Tom</style></author><author><style face="normal" font="default" size="100%">Mason, Tamara</style></author><author><style face="normal" font="default" size="100%">Vicente, Gina</style></author><author><style face="normal" font="default" size="100%">Dasilva, Michael</style></author><author><style face="normal" font="default" size="100%">Brodeur, Wendy</style></author><author><style face="normal" font="default" size="100%">DeSmet, Timothy</style></author><author><style face="normal" font="default" size="100%">Dodge, Sheila</style></author><author><style face="normal" font="default" size="100%">Lennon, Niall J</style></author><author><style face="normal" font="default" size="100%">Gabriel, Stacey</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms.</style></title><secondary-title><style face="normal" font="default" size="100%">BMC Genomics</style></secondary-title><alt-title><style face="normal" font="default" size="100%">BMC Genomics</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Library</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome, Human</style></keyword><keyword><style  face="normal" font="default" size="100%">High-Throughput Nucleotide Sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis, DNA</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2018 May 08</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">19</style></volume><pages><style face="normal" font="default" size="100%">332</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;BACKGROUND: &lt;/b&gt;Here we present an in-depth characterization of the mechanism of sequencer-induced sample contamination due to the phenomenon of index swapping that impacts Illumina sequencers employing patterned flow cells with Exclusion Amplification (ExAmp) chemistry (HiSeqX, HiSeq4000, and NovaSeq). We also present a remediation method that minimizes the impact of such swaps.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS: &lt;/b&gt;Leveraging data collected over a two-year period, we demonstrate the widespread prevalence of index swapping in patterned flow cell data. We calculate mean swap rates across multiple sample preparation methods and sequencer models, demonstrating that different library methods can have vastly different swapping rates and that even non-ExAmp chemistry instruments display trace levels of index swapping. We provide methods for eliminating sample data cross contamination by utilizing non-redundant dual indexing for complete filtering of index swapped reads, and share the sequences for 96 non-combinatorial dual indexes we have validated across various library preparation methods and sequencer models. Finally, using computational methods we provide a greater insight into the mechanism of index swapping.&lt;/p&gt;&lt;p&gt;&lt;b&gt;CONCLUSIONS: &lt;/b&gt;Index swapping in pooled libraries is a prevalent phenomenon that we observe at a rate of 0.2 to 6% in all sequencing runs on HiSeqX, HiSeq 4000/3000, and NovaSeq. Utilizing non-redundant dual indexing allows for the removal (flagging/filtering) of these swapped reads and eliminates swapping induced sample contamination, which is critical for sensitive applications such as RNA-seq, single cell, blood biopsy using circulating tumor DNA, or clinical sequencing.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/29739332?dopt=Abstract</style></custom1></record></records></xml>